Summary and Info
Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags.The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy.Content: Chapter 1 Fundamentals of Light Microscopy (pages 1–19): Chapter 2 Light and Color (pages 21–33): Chapter 3 Illuminators, Filters, and the Isolation of Specific Wavelengths (pages 35–51): Chapter 4 Lenses and Geometrical Optics (pages 53–77): Chapter 5 Diffraction and Interference in Image Formation (pages 79–101): Chapter 6 Diffraction and Spatial Resolution (pages 103–113): Chapter 7 Phase Contrast Microscopy and Darkfield Microscopy (pages 115–133): Chapter 8 Properties of Polarized Light (pages 135–151): Chapter 9 Polarization Microscopy (pages 153–171): Chapter 10 Differential Interference Contrast Microscopy and Modulation Contrast Microscopy (pages 173–197): Chapter 11 Fluorescence Microscopy (pages 199–231): Chapter 12 Fluorescence Imaging of Dynamic Molecular Processes (pages 233–263): Chapter 13 Confocal Laser Scanning Microscopy (pages 265–305): Chapter 14 Two?Photon Excitation Fluorescence Microscopy (pages 307–329): Chapter 15 Superresolution Imaging (pages 331–355): Chapter 16 Imaging Living Cells with the Microscope (pages 357–387): Chapter 17 Fundamentals of Digital Imaging (pages 389–413): Chapter 18 Digital Image Processing (pages 415–449):
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