Summary and Info
Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced. Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e. g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures. How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.Content: Chapter 1 Introduction (pages 1–13): Chapter 2 Precoated Layers (pages 15–33): Chapter 3 Before the TLC Development Process (pages 35–68): Chapter 4 Solvent Systems, Developing Chambers and Development (pages 69–112): Chapter 5 Evaluation Without Derivatization (pages 113–123): Chapter 6 Derivatization (pages 125–151): Chapter 7 Evaluation After Derivatization (pages 153–180): Chapter 8 Documentation (pages 181–201): Chapter 9 GMP/GLP?Conforming Operations in TLC (pages 203–239): Chapter 10 Effects of Stress (pages 241–245): Chapter 11 Special Methods in TLC (pages 247–252): Chapter 12 Appendix (pages 253–270):
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