Summary and Info
Distinguished scientists and researchers present a comprehensive collection of current preparative PCR techniques that can be used in cloning and modifying DNA and cDNA. Topics include performing and optimizing PCR (including long PCR), cloning PCR products, cloning unknown neighboring DNA, and library construction and screening. Also covered are mutagenesis, recombination, and in vitro selection, differential and subtractive approaches to cDNA analysis and screening, and cloning members of gene families. The techniques bring to both new and established researchers the power to apply PCR-based methodology to the cloning and modification of DNA, either through innovative protocols or by fostering individual creativity to modify and customize the protocols to best fit their own needs.
More About the Author
Bruce Whitehead is a legislator in the U.S. state of Colorado. Appointed to the Colorado State Senate as a Democrat in 2009, Whitehead represents Senate District 6, which covers much of south-western Colorado, encompassing Archuleta, Dolores, La Plata, Montezuma, Montrose, Ouray, San Juan, and San Miguel counties.
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